Abstract Frankliniella zucchini transmits zucchini lethal chlorosis virus, causal agent of lethal chlorosis of zucchini squash. The characteristics of relationship between this virus with its vector have not been studied, one of the reasons being the lack of a method for rearing the thrips for laboratory studies. This work proposes a system for the rearing of F. zucchini on fresh virus free zucchini ‘Caserta’ fruits, offering a practical and efficient alternative for the supply of a large number of insects for later study of virus/vector relationship. In addition, to aid in the identification of this species of thrips, the immature and adult forms obtained from the colony were described.

Tomato chlorosis virus (ToCV) é uma espécie do gênero Crinivirus, transmitida por Bemisia tabaci biótipo B e que foi primeiramente detectada no Brasil em 2006 no município de Sumaré, Estado de São Paulo. No período de 2007 a 2010, análises de RT-PCR seguida de "nested-PCR", com oligonucleotídeos iniciadores específicos para a detecção do ToCV e o sequenciamento dos fragmentos amplificados confirmaram a presença desse vírus em amostras de plantas sintomáticas de tomate (Solanum lycopersicum) provenientes de plantações em municípios dos estados da Bahia (BA), Espírito Santo (ES), Goiás (GO), Minas Gerais (MG) e Rio de Janeiro (RJ). Este é o primeiro relato do ToCV nos Estados da BA, ES, GO, MG e RJ, sugerindo uma ampla disseminação desse vírus no Brasil.

Tomato chlorosis virus (ToCV) is a species in the genus Crinivirus, transmitted by Bemisia tabaci biotype B, first detected in Brazil in 2006 in the county of Sumaré, State of São Paulo. During the period from 2007 to 2010, RT-PCR analyses followed by nested-PCR with specific primers for ToCV detection, and sequencing of the amplicons, confirmed the presence of this virus in samples of symptomatic tomato plants (Solanum lycopersicum) from crops in counties in the states of Bahia (BA), Espírito Santo (ES), Goiás (GO), Minas Gerais (MG), and Rio de Janeiro (RJ). This is the first ToCV report for the states of BA, ES, GO, MG, and RJ, suggesting that this virus is widely disseminated in Brazil.

Avaliou-se a transmissão de Passion fruit woodiness virus (PWV) por Aphis gossypii (Glover). Em dois experimentos independentes, o afídeo transmitiu o PWV para maracujazeiros com taxas de 75% e 100%, ao se depositarem oito e doze afídeos virulíferos por planta, respectivamente. No final dos testes, observaram-se, em algumas plantas de maracujá, formas ápteras e ninfas de A. gossypii, sugerindo a colonização dessas plantas pelo afídeo. Esse parece ser o primeiro relato da colonização de Passiflora edulis f. flavicarpa (Deneger) por uma espécie de afídeo.

The transmission of Passion fruit woodiness virus (PWV) by Aphis gossypii (Glover) was evaluated. In two independent experiments, A. gossypii transmitted PWV to passion fruit plants at the rates of 75% and 100%, when eight and twelve viruliferous aphids were deposited by plant, respectively. At the end of the tests, nymphs of A. gossypii were observed in some of the passion fruit plants, suggesting that the aphid species was colonizing the plants. This seems to be the first report of Passiflora edulis f. flavicarpa (Deneger) colonization by a species of aphid.